Monday to Friday 8am - 5pm GMT. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. Never order or replace filters to optimize an assay again!SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 0 × 107) RLU 0. Superior optics Microplate Reader Shelf. ELISA Plate Readers / Microplate Readers. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. You can also contact Technical Support by email. 13. Retrieving Each. To update the driver. This document is provided to customers who have purchased Molecular Devices, Inc. 0%, raw data units = counts/sec. A plate reader’s software may offer automated analysis using protocols configured for a particular assay. Used. SoftMax Pro 7. Plate stacker and robot integration The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within minutes and begin reading microplates with seven mouse clicks. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. 1. Molecular Devices. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. An industry-renowned instrument for monitoring GPCRs and ion channels. Select Start>Control panel>Device Manager. Select the Driver tab and click Update Driver. absorbance plate readers. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. Microplate Stacker and Washer. • 0. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. Choose the desired plate type, using the upper dropdownThe Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. It was then read from the bottom on a SpectraMax ® i3 Multi-Mode Microplate Reader using a preconfigured protocol in SoftMax Pro Software with the settings indicated in Table 1. 1. Next generation SpectraMax® absorbance microplate readers. Contact our support team with your questions regarding Molecular Devices products and applications. ELISA (Enzyme-linked Immunosorbent Assay) is one of the most popular quantitative methods to detect a target antigen such as toxin or foreign substance within a sample. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax:. 4: Test tube cover. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. #ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. Select the Driver tab. the iD5 reader’s filter system. Sale price $18,499. To contact technical support by phone in the U. Wait 20 seconds. 313 reference gain check fail. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. 4: Test tube cover. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Figure 10: The Plate section Template Editor, showing the series set up. The Versamax Microplate Reader is built around a grating monochromator. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. 0. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Robust, high value microplate reader designed to deliver research-grade results to any laboratory. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. #iD3) Methods. #0200-7011) Instrument setup SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Unplug the power cable from the back of the instrument. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A)Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. 0200-6263. 5 µL of each sample was pipetted into a 96-well. 4: Test tube cover. Learn how Molecular Devices FlexStation 3 Multi-Mode Microplate Reader provides life science industry solutions. S. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. What plates do you recommend for SpectraMax Plate Readers? Luminescence : Solid white plates for top reads and white plates with clear bottoms for. With optimized reagents, option to operate the. Clone Screening. Read times. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. These robust readers have been placed in labs from. Restart the system. Compact designs. Microplate Stacker and Washer. . ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. , target CV per well = 1. Superior optical system includes a xenon flash lamp and features. 1. 96-well plate and 12,500-20,000. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. 3860 N First Street San Jose, CA 95134. For SpectraMax ABS Plus reader, r 2 = 0. 190. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Search. Note: removal of medium, followed by addition of a 1X solution of calcein AM and EthD-III, is. please click here. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. With SoftMax Pro Software, the PathCheck Sensor automatically normalizesThe SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Important things to consider when purchasing a multimode microplate reader. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. After reading the ELISA plate on both readers, each standard curve was nearly identical. Table of Contents. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. The plate reader optics are tunable for absorbance, fluorescence intensity,. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. Instrument settings can be saved as a protocol file. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. 00. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. For more sample throughput, the system can be easily integrated into a full roboticOnly Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Absorbance Plate Reader Comparison Chart. Fluorescence Microplate Reader Comparison Chart. 9000-0756. Mode Reader with the StakMax Microplate Handling System from Molecular Devices. 1. Uncontrolled Copy for Training and Reference Purposes Only . SoftMax® Pro 7. Molecular Devices products are warranted to meet the stated specifications. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Plus 384. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. was added and absorbance was measured at 450 nm and 650 nm using a plate reader (Molecular Devices, SpectraMax ABS Plus, SoftMax Pro 7. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The FlexStation® Scanning Fluorometer (Molecular Devices; Fig. Tristar 3 Multimode Microplate Reader. 더 많은. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Videos & Webinars. The FlexStation 3 reader exclusively offers fluid transfer validation using Molecular Devices patented PathCheck® Feature to quantify the integrated 8- and 16-channel pipettor head performance. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. These systems are hybrids that allow you to choose between. and it is unrealistic to expect one experiment to occupy a plate reader for multiple hours or days. 2. 5: Schematic of the back panel of a reader. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. In addition, users can define individual reagents and concentrations to be delivered to each well. Shortside barcode reader: 0310-5102. , call 1-800-635-5577. The guide also covers the software features, the specifications, and the accessories of the systems. ORDER. The direct transfer capability reduces We would like to show you a description here but the site won’t allow us. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. 100 μL of blank or endotoxin standard (in. Get a front row view of your cells. The VMax® Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm–750nm), with 4 filter choices included at time of purchase. From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. Fluorescent Imaging Plate Reader (FLIPRTM ) technology to measure Ca2+ flux in cells was an important development in the early 1990’s and has played a significant role in. Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast,. HTRF-compatible SpectraMax Multi-Mode Readers. Instrument settings can be saved as a protocol file. Molecular Devices SpectraMax Gemini XS Microplate Reader. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Microplate Reader Shelf. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). As new assays are developed or your needs change, simply add a detection cartridge like ScanLater™ Western Blot System or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system. Endpoint. For customers outside the U. Microplate readers and controlling software enable walk away data analysis. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. VIS absorbance detection. Under "Category", select "Plate Type". To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Fig 2: Enzymatic response of synthetic compounds. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Beyond the hardware, a microplate reader’s software also impacts performance and flexibility. In an analogous manner, the same sample names were assigned to a CuvetteSet section. The EMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. in steps of 0. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmFor the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. 40266 Email: dru[email protected] iD5 reader’s filter system. Set up the instrument and software. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. APP Automated. together with a Molecular Devices plate. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. 5: Schematic of. 0 . The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 314 low lamp level warning. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. Clone Screening. Are you looking for a versatile and affordable microplate reader that can perform various assays and measurements? Check out the FilterMax F3 and F5 Multi-Mode Microplate Readers, which offer absorbance, fluorescence, luminescence, and more read modes with high performance and reliability. Fluorescence analysis was performed using a SpectraMax iD3 multi-mode plate reader (Molecular Devices LLC, U. For biochemical assays, the washer removes unbound material and unreacted reagents. 1. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Your applications, your modes, your choice. gene expression with a user-friendly reader. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Consider non binding surface type plate (Greiner and Corning are 2 manufacturers)Molecular Devices microplate readers. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. TheUnparalleled performance on a personalized platform. D. 997 (n = 3). SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. An ampicillin-resistant E. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. SpectraMax Absorbance, Fluorescence. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. . The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular. V. visible absorbance, fluorescence. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Optimize. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Here, the CVs for each plate were less than 3, and the difference between mean onset time for the plates run on the two readers was only 7%. With The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Multimode Microplate Readers. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. The opaque walls prevent stray light from being detected from neighbouring. First, as mentioned before, the walkaway time significantly. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. The SpectraMax ABS Plus reader utilizes Molecular . SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Step 1: Turn on your microplate reader. Plate pre-read measurements were made in a Costar UV plate. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Instrument settings can be saved as a protocol file. 0. The plate is set up for reading and images are acquired according to specified parameters. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Explore our fully-integrated,. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. One plate carrier adapter is provided with the instrument. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. Also refer to the Multimode Reader Comparison Chart Features. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 0 software Download page. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of the human. Maintaining data integrity is a. The test compound menadione was added to cells in a 1:3. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. These systems are hybrids that allow you to choose between. Reader Documentation Version No. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. We would like to show you a description here but the site won’t allow us. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. 1-1. Download PDF versions of the SpectraMax Microplate Reader User Guides. From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. 1). Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. It features a modular design that allows users to add or change detection cartridges according to their needs. The two different data import options are tailored to quickly get your data into SoftMax Pro to provide a single source of software for all your data reduction needs. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. This five-mode microplate reader with automatic filter identification and western blot capability is the complete laboratory solution to help scientists increase. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre. Multi-mode Plate Reader. Item SpectraMax i3x Multi-Mode Microplate Reader. Avoid losing high signal. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. The PyroGene assay has been optimized to be linear from 0. A control well was set up to verify the accuracy of the standard curve. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Perkin Elmer Victor X2 Multimode Microplate Plate Reader 2030-0020 w/ PC. Plate reader. Three low volume 24-well bottom slides. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Some familiarity with Windows should be sufficient to use it. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Remove the lamp screw (on the right side of the lamp, as shown in the diagram) then gently pull the connecting wires to remove the lamp from the body of the instrument. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 9. D 1. SpectraTest® Validation Package User Manual Download Page. Catalog Number i3x. Select the Driver tab. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. . Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The plate reader optics are tunable for absorbance. 0A Sunnyvale, CA Made in USAFilter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Temperature control and shaking come standard. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. If no sound is heard during initialization, contact technical support. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 0. SpectraDrop High-Throughput Screening (HTS) Kit. Open the top cover of the instrument. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Lab automation for high-throughput plate-based assays. Learn how you can utilize microplate readers to drive landmark discoveries. 635. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Plate. StakMax Handling Stacker Base Unit. S. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 00 USD. With optimized reagents, option to operate the. FlexStation® 3 Multi-Mode Microplate Reader. Description Figure 1. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Molecular Devices' microplate readers. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. 1 - 16 of 69. Monday to Friday 7am - 5pm Pacific Time. Prior to fluorescence analysis, the lid from the OmniTray plate was removed. Table of Contents. Integrate . A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. Microplate Reader Comparison Charts Published Dec 16, 2020 | Updated Dec 16, 2020 Products : General, FilterMax F5 reader Please click on the links below to. STAKMAX. Lastly, we demonstrate the utility of this novel fluorescence-based assay to evaluate the activities of. 1. Step 3: Click “Settings” and a screen will appear (Figure 1). 4 °C above ambient to 45 °C. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. July 17, 2023. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Label-free detection. Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. PathCheck sensor corrects optical pathlength & pipetting variation. Instrument settings can be saved as a protocol file. In this product video, see how the SpectraMax M5 quickly reads samples. Feb 22, 2023Used. Turn the reader on and secure the USB cable. FLIPR Calcium 4 Assay Kit. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). Cytomat 9 Hotel (MPH) BAR CODE READING: FluidX: Perception (Vial Rack Bottom Label 2D Reader) Microscan: MS-3 Laser Bar Code Reader: Thermo Fisher Scientific: VisionMate 96 (Vial Rack Bottom Label 2D Reader). The instrument settings were identical to those used in Example 1 above. ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. SpectraMax MiniMax 300 imaging cytometer enables quick imaging and analysis of cells and helps in observing the viability of your cells with the field-upgradeable cellular imaging for spectramax i3/i3x multi-mode detection platform. Page 1 of 8 . The Back Panel Figure 1. Gregory et al. #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. AXON Patch-Clamp. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. Standard features include a cuvette port,. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. The adapter is required for optimum performance with standard 96-well and 384-. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Cells were thawed and propagated according to the recommended method specified by the supplier. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Description: ScanLater* Western Blot Detection Cartridge, enables the first western blot detection capability to a microplate reader, Time-resolved fluorescence detection, Xenon flash lamp excitation source. Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Import plate-based data from any vendor SoftMax Pro enables you to import data from acquisition sources other than a Molecular Devices microplate reader. SoftMax Pro 7. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. Lamp source is a Xenon flash lamp (1 Joule/flash). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. Integrated stacker option. Plate Formats: 6 to 384-well microplates. VMax . Please contact your Molecular Devices representative for configuration options.